Accessories for CoolLED-Light Engines

Description

CoolLED offers a range of options and accessories for use with its LED illumination systems. These complement and enhance the performance of controlled light delivery to a microscope or sample in a number of ways. Products include combiners, dichroic mirrors, filter holders and microscope adaptors. Select a tab below for the various product categories.

Combiners:

The pE-Dual Adapt allows two illumination systems to be connected physically to the fluorescence port of the microscope at the same time. This enables users to choose between LED illumination or mercury/metal-halide without having to exchange individual source and re-align them on the microscope.

Any CoolLED illumination system can be fitted to the port at 90 degrees with the mercury or metal halide attached to the in-line port. Switching between illumination systems is simply achieved by moving a mirror between 0 and 45 degrees with a lever. The in-line port has an additional manual shutter to prevent the white light from a mercury or metal halide illuminator causing interference when the CoolLED LED illumination system is being used.

pE-Combiners are used to combine two CoolLED illumination systems into a single optical path.  A number of pE-Combiners can be used to support multiple systems on a microscope

An appropriate dichroic mirror is selected in each case to match the LED wavebands in the illumination systems.  This is an excellent configuration for dual staining applications.

The pE-Combiner can be purchased as a package with wavelength-defined pE-100 illumination systems.  An advanced version is available with excitation filter holders for users who wish to filter the excitation light before it enters the microscope or is delivered to the sample.

Adaptors:

A Microscope Adaptor is normally specified at time of supply of the illumination system. Additional adaptors can be purchased when the system is to be transferred to another microscope. You will need to re-align the illumination system to the optical path of the new microscope. This is a simple task which will only take a few minutes. pE-100 illumination systems are currently supplied with a fixed adaptor and the light source must be returned to CoolLED to make this change. All other CoolLED illumination systems use exchangeable microscope adaptors.

Dichroics:

A Dichroic Mirror is required to combine different channels of light into a single optical path and is selected in each case to match the corresponding LED wavebands in the CoolLED illumination system.

When the LED wavebands are exchanged, it may be necessary to change the dichroic mirror at the same time depending on the new wavelengths installed in the system. This item is used within the pE-Collimator and the pE-Combiner.

Filter holders:

Excitation Filter Holders are used in the pE-4000, pE-2, pE-Combiner and some pE-100 systems.  They allow the user to insert standard 25mm diameter excitation filters into the optical path of the illumination system.  The benefit is that the excitation light can be filtered before it is delivered to the microscope or sample.  Filter wheels are no longer required on a microscope and a narrower waveband of excitation light delivered to a sample.

LAMs:

The modular pE-2 illumination system can accept interchangeable LAMs which the user can purchase and replace depending on the required wavelength for a specific experiment.  The pE-2 Light Source will automatically recognise the newly installed wavelengths and read the drive parameters for each wavelength.  The control pod will display the installed LED wavelengths.

Communication:

The pE-Breakout Box is designed for use with the pE-2 illumination system and connects directly to the 15-pin D-type connector at the rear of the pE-2 Light Source.  BNC connections allow the user to connect to control software for independent wavelength triggering.

The rear mounted pE-Expansion Box is a powerful accessory for the pE-4000 illumination system.  It fits directly to the rear of the pE-4000 Light Source and has multiple BNC connections for remote Analogue and TTL control with synch-in and synch-out capability.

Collimation:

CoolLED’s pE-Universal Collimator delivers collimated light from a 3mm diameter liquid light guide to almost any microscope. It has two independently set optimisation parameters that can be configured for individual performance on all current and most older microscopes.

CoolLED´s pE-340fura is configurable to accept a particular UV compatible 3mm liquid light guide, which will ensure high transmission of the 340nm wavelength.  An optional pE-340fura Universal Collimator is available if required.